Evaluation of Saudi Fluorescent Pseudomonads Isolates as a Biocontrol Agent against Citrus Canker Disease Caused by Xanthomonas citri subsp citri A*
Mohammed
Al-Saleh
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Kingdom of Saudi Arabia
author
text
article
2014
eng
The goal of this study was to determine whetherbacterial antagonists could be used to control Xanthomonas citri subspcitri (Xcc), the causal agent of bacterial citrus canker disease. Atotal of 22 potentially bacterial antagonists isolated as epiphytes from thephylloplane of healthy citrus trees were screened for their in vitro biologicalcontrol capability against Xcc. These strains were identified as Pseudomonas fluorescens onthe basis of biochemical and physiological tests and 16S rDNA. Out of these 22potentially bacterial antagonists, five strains (KSA1, KSA9, KSA14, KSA17, andKSA20) showed high potential growth inhibition capabilities against Xcc.The KSA1 strain was selected for further studies to test its in vivocapability to control bacterial citrus canker pathogen. It was sprayed in asuspension of 107CFU ml-1on citrus leaves which were subsequently inoculated after 72 h with 108 CFU ml-1 suspension of Xcc strain JQ890095.According to the in vivo biocontrol tests, the putative antagonist KSA1significantly reduced the symptoms on the leaves of Mexican lime seedlingscompared with untreated inoculated plants.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
1
7
https://eajbsg.journals.ekb.eg/article_16493_34b0f73acfc22469c117dbab8c504fd0.pdf
dx.doi.org/10.21608/eajbsg.2014.16493
New Records of Marine Algal Species Collected From Some Localities of Al-Jabel Al-Akhdar Coastline, Libya
Magda
El-Adl
-Botany Department, Faculty of Science, Damietta University, Damietta, Egypt.
-Botany Department, Faculty of Education (Al-Qubba), Omar Al-Mukhtar University, Al- Beidaa, Libya.
author
text
article
2014
eng
Fieldsurveys were planned to screen and document the new macroalgal species ofAl-Jabel Al- Akhdar coastline. Seven different sites (S1-S7) of the target areawere randomly and independently visited during remittent times between October2012 and April 2013 for collecting samples. Of 12 new record algal species, 6were recorded for the first time for the Libyan algal flora; 3 for Al-Jabel Al-Akhdar coastline and 3 for the investigated area. These 6 newly record algalspecies for the Libyan environment were Vaucheria piloboloides Thuret (Heterokontophyta), Penicillus dumetosus (Lamouroux) Blainville, Cladophora laetevirens (Dillwyn)Kützing (Chlorophyta), Corallina pilulifera Postels & Ruprecht, Champia parvula (Agardh) Harvey and Thuretellaschousboei (Thuret)F. Schmitz(Rhodophyta). The physico-chemical analysis indicated that, Ch. parvula,C. pilulifera, Bangia atropurpurea (Maertensex Roth) C. Agardh, LithophyllumincrustansPhilippi, Caulerpa racemosa var. cylindracea (Sonder) Verlaque, Huisman et Boudouresque and Chaetomorphalinum (O.F. Müller) Kützing were found to be restricted to pollutedseawater while Cl. laetevirens inhabited both sites (as a pelt in theclean site and patches in polluted site).Water quality of the target area wasclassified as clean (oligotrophic), ranging between mostly very clean (ultraoligotrophic)in S4 and S7 and moderate polluted (mesoeutrophic) in S3 and S6. Most exotic species were found to belong to Rhodophyta. The present study recommended further surveysto explore the newly Libyan algal species.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
9
20
https://eajbsg.journals.ekb.eg/article_16494_a5806d0e2ab2c3d4392e48148e0a4326.pdf
dx.doi.org/10.21608/eajbsg.2014.16494
Prevalence of Human Papilloma Virus subtypes 52,56,58,59 and 66 among Yemeni Patients with Cervical Cancer
Saleh
Bensumaidea
Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Sudan
author
Salim
Bafakeer
Department of Pathology, College of Medicine, Hadhramout University of Science and Technology, Yemen
author
Mohammed
Abd El Aziz
Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Sudan
author
Fawaz
Alshammari
Department of Medical Laboratory, College of applied Medical Science, University of Hail.
author
Hussain
Ahmed
Department of Pathology, College of Medicine, Molecular Diagnostics and Personalized Therapeutics Unit, University of Hail, KSA; Department of Histopathology and Cytology, FMLS, University of Khartoum, Sudan
author
text
article
2014
eng
Background: Knowledge of high risk Human Papilloma Virus (HPV) subtypes might be helpful for development of strategies for decreasing the burden of risk of cervical cancer. Therefore, the aim of this study was to screen for some HR-HPV subtypes that are less common in many regions.
Methodology: A total of 150tissue samples obtained from patients with cervical cancer in addition to 50 tissue samples obtained from patients with benign cervical lesions, were investigated for the presence of HPV subtypes 52,56,58,59 and 66 by Polymerase Chain Reaction (PCR).
Results: The prevalence of HPV subtypes 52,56,58,59, and 66, among cases was 0.6%, 0%, 4%, 3.3% and 0% respectively.
Conclusion: HPV subtypes 58 and 59 have a considerable contribution to etiology of cervical cancer in Yemen that requires further consideration.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
21
26
https://eajbsg.journals.ekb.eg/article_16495_10f9f4d6ab93dfbd1c02fac8d552f2c2.pdf
dx.doi.org/10.21608/eajbsg.2014.16495
Is Herpes Simplex Virus (HSV) infection a risk factor for nasopharyngeal carcinoma?
Hussain
Ahmed
Department of Pathology, College of Medicine, Molecular Diagnostics and Personalized Therapeutics Unit, University of Hail, KSA; Department of Histopathology and Cytology, FMLS, University of Khartoum, Sudan
author
Rania
Suliman
Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Sudan
author
Mohammed
Abd El Aziz
Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Sudan
author
Fawaz
Alshammary
Department of Medical Laboratory Science, College of Applied Medical Science, University of Hail, KSA
author
Faris
Altom
Department of Medical Laboratory Science, College of Applied Medical Science, University of Taibah, KSA
author
Ibrahim
Ginawi
Department of Pathology, College of Medicine, Molecular Diagnostics and Personalized Therapeutics Unit, University of Hail, KSA; Department of Histopathology and Cytology, FMLS, University of Khartoum, Sudan
author
text
article
2014
eng
Objective: The aim of this study was to screen for the presenceof HSV in patients with nasopharyngeal Carcinoma (NPC). Methodology:Formalin fixed paraffin wax processed NPC tissue were obtained from 150 tissueblocks and retrospectively investigated for the presence of HSV-1 and HSV-2using polymerase Chain Reaction (PCR). Results: Of the 150 NPC tissuespecimens, Herpes Simplex Virus type 2 (HSV-2) was identified in 18/150 (12%)samples. Out of the 18 samples infected with HSV-2, 15/97 (15.5%) among malesand 3/53(5.7%) were among females. Conclusion: The present have shownsome weak links between HSV and NPC. The great majority of samples harboringHSV were also found to harbor EBV, which suggests the potentiality of EBV overHSV.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
27
32
https://eajbsg.journals.ekb.eg/article_16496_7445d1cac1185e1e462526d422428e46.pdf
dx.doi.org/10.21608/eajbsg.2014.16496
Assessment of Infectious and Inflammatory changes in sputum associated with cement dust
Fawaz
Alshammari
College of Applied Medical Science, University of Hail, KSA
author
Bakr
Hommi
Sudan University for Sciences and Technology, Sudan
author
Hussain
Ahmed
College of Medicine, university of Hail, KSA.
author
text
article
2014
eng
Background: sputum cytology is an important biomarker that can be used to assess infectious and inflammatory changes associated with different etiological factors. Therefore, the objective of this study was to assess the infectious and inflammatory changes associated with exposure to cement dust.
Methodology: In this cross-sectional cases control study, 500 apparently healthy volunteers were selected. Of the 500 study subjects, 400 were exposed to cement dust (ascertained as cases) and 100 were non-exposed (ascertained as controls). Sputum specimen was collected and subsequently demonstrated using Papanicalauo method.
Results: Inflammatory cells infiltrate associated with exposure to cement dust was found to be statistically significant (RR= 2.1214, 95% CI = 1.6142 to 2.7881, P < 0.0001). The risk of viral infection associated with exposure to cement dust was found to be (RR= 1.6250, 95% CI = 0.7078 to 3.7308, P = 0.2522). The risk associated with bacterial infection was (RR= 13.6350, 95% CI = 1.9091 to 97.3805, P = 0.0092). The risk associated with fungal infection was (RR= 2.2725, 95% CI = 0.2912 to 17.7315, P = 0.4335).
Conclusion: Exposure to cement dust is a significant risk that enhances infectious and inflammatory change in the lung of exposed individuals.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
33
40
https://eajbsg.journals.ekb.eg/article_16498_e28a8140ac22871b4004c43baac61cd1.pdf
dx.doi.org/10.21608/eajbsg.2014.16498
Effects of Allergic fungi on hematological and immunological parameters of human patients and rabbits
Sanaa
Mohamad
Biology Department, College of Science, Kirkuk University, Iraq
author
Karkaz
Thalij
Food Science Department, College of Agriculture, Tikreet University, Iraq
author
Khalil
AL-Bander
Biology Department, College of Science, Tikreet University, Iraq
author
Batol
Dheeb
Biology Department, College of Science, Iraqia University, Iraq
author
text
article
2014
eng
Thehematological parameters and immunoglobulin levels were estimated in patientsand healthy controls as well as experimentally infected lab animals. Rabbitswere infected with fungi (A. niger, C. albicans, A. fumigatus, A. flavus,Fusarium spp, Alternaria spp). For 21 days before serum collection. Two hundred serum samples were collected from the respiratorytract infected patients and 20 samples from age and sex matched healthypersons. Hematological parameters in human and rabbits indicated highsignificance (p<0.001) of most blood parameters compared with control groupsincluding leukocytosis; lymphocytosis; eosinophilia and increased ESR. ELISAtestsshowed highly significant levels of IgE and IL4 (P≤0.001) in patientscompared with the healthy people, and the highest increased were with thefungus Aspergillus fumigatus (450 IU /ml; 15pg / ml). Similarly, thelevels of IgE and IgM in rabbits inhaled with fungi had significantly increasedto 425 IU /ml, 390 mg/dl with Aspergillus fumigatus.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
41
48
https://eajbsg.journals.ekb.eg/article_16499_01182e0e8fe9cfb3ac1250e81948156d.pdf
dx.doi.org/10.21608/eajbsg.2014.16499
Isolation and Identification of Mycoflora Contaminated of Yemeni Coffee Beans
Humaid
A.
Biology Department, Faculty of Science, Sana'a University, Sana'a, Yemen
author
Alghalibi
S.
Biology Department, Faculty of Science, Sana'a University, Sana'a, Yemen
author
Al-khalaqi
A.
Biology Department, Faculty of Science, Sana'a University, Sana'a, Yemen
author
text
article
2014
eng
Thiswork was designed to study the mycoflora of stored Yemeni green and roastedcoffee beans, in 50 samples (25 samples each) which were collected randomlyfrom different markets in Sana'a city, Yemen during 2013 using directplating technique. Our results showed that in the individual samples of greenand roasted coffee beans, the counts of fungi were ranging from 110 to 236 CUF/ 100 beans and 8 to 249 CUF/100 beans, respectively. As well the broadestspectrum of fungal species were 55 species and 3 varieties belonging to 13genera and 66 species and 3 varieties belonging to 23 genera in case of greenand roasted coffee beans, respectively. Aspergillus, Penicillium, andRhizopus followed by Fennelliaand Fusarium were the mostcommon genera isolated from both the two types of coffee beans samples, whereasEurotiumwas the most common genus only in roasted coffee bean samples,while it was absent in green coffee beans samples.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
49
58
https://eajbsg.journals.ekb.eg/article_16500_7f594ef42822428ef87d4b6fbd47513d.pdf
dx.doi.org/10.21608/eajbsg.2014.16500
Antibacterial activities of crude extracts of Nigerian spices and herbs on enteropathogens
Janet
Olaitan
Department of Biological Sciences, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Osun State, Nigeria.
author
Olufunke
Shittu
Department of Microbiology, College of Natural Sciences, Federal University of Agriculture,
author
text
article
2014
eng
Aim: This study was designed to evaluate the antibacterial activities of water and ethanolic extracts of Nigerian herbs and spices against enteric pathogens.
Methodology: Crude extracts of fresh leaves of Ocimum gratissimum, Psidium guajava, Vernonia amygdalina, Kigelia africana, Azadrichata indica, Pistia stratiotes, Euphobia hirta;bulb of Allum sativum and rhizomes of Zingiber officinale and Curcuma longa were evaluatedusing the agar diffusion assay method and minimal inhibitory concentration (MIC) to determine the antibacterial activities against Vibrio cholerae, Escherichia coli and Salmonella sp. Gentamicin (28 mg/ml) was used as antibiotic control.
Results: Antimicrobial sensitivity test indicated that while Salmonella sp. was not inhibited by ethanolic extracts of Euphobia hirta and Curcuma longa, both water and ethanolic extracts of others inhibited the growth of Vibrio cholerae, Escherichia coli and Salmonella sp. to varying degree. The minimal inhibitory concentrations (MIC) determined for the crude extracts of the various herbs and spices, using water and aqueous ethanol inhibited the test isolates at high dilutions.
Conclusion: This study has shown the antibacterial potential of the herbs and spices against the tested enteric pathogens. The antibacterial activities of all the herbs and spices observed in this study justify their use in alternative medicine.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
59
68
https://eajbsg.journals.ekb.eg/article_16501_e9b689d47b41ea299724e454fead5519.pdf
dx.doi.org/10.21608/eajbsg.2014.16501
Evaluation Of Antimicrobial And Antioxidant Activities Of Some Edible Mushrooms
Ghoneimy
A.
Department of Botany and Microbiology , Faculty of science (Girls branch), Al Azhar University, Cairo, Egypt
author
Wu
A.
Department of Biology, Virginia Commonwealth University, Richmond, VA, USA
author
Maie
Elkhawaga
Department of Botany and Microbiology , Faculty of science (Girls branch), Al Azhar University, Cairo, Egypt
author
Alassar
M.
Department of Botany and Microbiology , Faculty of science (Girls branch), Al Azhar University, Cairo, Egypt
author
Abdelaziz, M.M.
Abdelaziz, M.M.
Regional Center for Mycology and Biotechnology, Al Azhar University, Cairo, Egypt
author
Elbatrawy
N.
Regional Center for Mycology and Biotechnology, Al Azhar University, Cairo, Egypt
author
text
article
2014
eng
Fruitbodies, mycelial extracts and broth filtrate of five edible mushrooms namely Pleurotusostreatus, Pleurotus sajor caju, Agaricus campestris, Agaricusbisporous and Grifola frondosa have been evaluated for theirantimicrobial and antioxidant activities. Different solvents including water,ethanol, ethyl acetate, acetone, chloroform, and hexane were used to extractantimicrobial and antioxidant compounds of different mushrooms underinvestigation. The extracts were evaluated for their antimicrobial activitiesusing the agar diffusion method against some pathogenic bacteria and fungi. Theresults revealed that the aqueous extract of A. bisporous fruit bodyexhibited the highest antimicrobial activity in comparison to all other solventextracts. The antioxidant activities were analyzed using DPPH radicalscavenging, reducing power and the total phenolic assay. Results revealed that also aqueous extract ofP. sajor caju and Grifola frondosa fruit bodies exhibited thehighest antioxidant activity. Fractionation of the aqueous crude extracts of A.bisporous, P. sajor caju and G. frondosa fruit bodies producingthree purified bioactive proteineous compounds which have antimicrobialactivities for the first mushroom and antioxidant activities for latter testedmushrooms.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
6
v.
2
no.
2014
69
82
https://eajbsg.journals.ekb.eg/article_16502_36453e8ec7045d21844660f0f6bcab0b.pdf
dx.doi.org/10.21608/eajbsg.2014.16502