Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia africana.
Al-mahi
Idris
Faculty of Science, University of Khartoum. P.O. Box 321, Postal Code 11115, Khartoum, Sudan
author
Ietidal
Al-tahir
Faculty of Science, University of Khartoum. P.O. Box 321, Postal Code 11115, Khartoum, Sudan
author
Eihab
Idris
Faculty of Science, University of Khartoum. P.O. Box 321, Postal Code 11115, Khartoum, Sudan.
author
text
article
2013
eng
Theaim of this study was to identify the endophytic fungi of the medicinal plant Kigeliaafricana (Lam) Benth. (Bignoniaceae) and to investigate their potentialantimicrobial activity. Seven species of endophytic fungi were successfullyisolated from K. Africana for the first time: including Cladosporiumsp., Aspergillus flavus, Aspergillus sp., Curvularia lunata aswell as three unknown species. The fungal extracts were assessed for antibacterialactivity against three standard pathogenic bacterial strains: Bacillus subtilis, Staphylococcus aureus and Escherichia coli. Mostof the extracts showed in vitro inhibition of bacterial growth. Thephytochemicalscreening revealed the existence of a diverse group of secondary metabolites inthe crude extracts of the endophytic fungi that resemble those in the hostplant extracts.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
5
v.
1
no.
2013
1
9
https://eajbsg.journals.ekb.eg/article_16639_faa4558b14e9674ac116114d04b184de.pdf
dx.doi.org/10.21608/eajbsg.2013.16639
Mycobacterium avium subspecies paratuberculosis in Camels; Clinical Aspects and Control Suggestions
Ghanem
Al-Ghamdi
Department of Biology, College of Science, Al-Baha University, Al-Baha, Saudi Arabia
author
text
article
2013
eng
Camel paratuberculosis which is caused by Mycobacterium avium subspecies paratuberculosis, is a chronic granulomatous enteric disease. Several domestic and wild ruminants species may be affected by this organism which complicates the epidemiology of the disease. The clinical manifestation and control measurements of the disease in the camel is not well characterized. The aim of this article is to explore the clinical manifestations and the possible strategies to control the disease in camels.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
5
v.
1
no.
2013
11
15
https://eajbsg.journals.ekb.eg/article_16640_962ba359a83442b5e5db2fc4ae376a0c.pdf
dx.doi.org/10.21608/eajbsg.2013.16640
Increasing prevalence of ESBL-producing Enterobacteriaceae in Sudan community patients with UTIs
Omar
Ahmed
Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute for Hajj and Omraa. Umm Alqura University, Makkah, Kingdam of Saudi Arabia
author
Alfadel
Omar
College of Medical Laboratory Science, Al Nilain University, Khartoum, Sudan
author
Atif
Asghar
Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute for Hajj and Omraa. Umm Alqura University, Makkah, Kingdam of Saudi Arabia
author
Mogahid
Elhassan
- Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Al Madena Al Monawarah, KSA.
author
text
article
2013
eng
Three hundred and thirty two (n=332) urine specimens were collected from patients attending different hospitals in KhartoumState during the period from May to November 2011. The isolates were transported to the microbiology lab (research lab) at SudanUniversity for Sciences and Technology. Identification of isolates was done by using conventional biochemical methods and microbact 2000 24E (OXOID) system. All Isolates were then stored at –70°C in Tryptic Soy broth with 20% glycerol. The isolated bacteria were tested for their antimicrobial resistance. ESBL screening was confirmed bydouble disk synergy test. The results showed that urinary Gram negative bacteria was 65.7%. High rate was caused by E. coli (72.0%) followed by K. pneumoniae (14.7%). Maximum sensitivity was seen for imipenem (100%), followed by piperacillin/tazobactum (91.7%) and cefoxitin (87.2%). The maximum resistance was seen against ceftazidime (74.8%) followed by cefotaxime (70.6%). ESBL producing bacteria was (59.6%) mostly were in K. pneumoniae (68.8.%) followed by E. coli (65.0 %). ESBL producing bacteriashowed maximum resistance to ceftazidime (95.4%), followed by cefotaxime (94.6%), while minimum resistance was seen with imipenem (0%), followed by piperacillin/tazobactam (3.8%) and cefepime (7.7%).
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
5
v.
1
no.
2013
17
24
https://eajbsg.journals.ekb.eg/article_16641_199ca46a2447692c95aa617e714fa806.pdf
dx.doi.org/10.21608/eajbsg.2013.16641
Screening of some synthetic fused heterocyclic pyrimidines for anti-avian influenza virus (H5N1) activity
Omar
Alfarouk
- Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
author
Ahmed
Barakat
- Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
author
Sahar
Shoman
Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
author
Husam
Ghanem
Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
author
Mohamed
Ali
Department of water pollution, National Research Center, Dokki, Cairo, Egypt
author
Aymn
Rashad
Department of Photochemistry, National Research Center, Dokki,Cairo, Egypt
author
text
article
2013
eng
Thirteen chemical compounds belonging to the fused heterocyclic pyrimidines were synthesized and screened in order to assay their antiviral activity against the avian influenza virus (H5N1) A/Chicken/Qalubiya/1/2006(H5N1) by plaque reduction assay on MDCK cells. All tested compounds were non-toxic on the cells. Ten out of thirteen compounds (10, 13, 9, 12, 1, 11, 7, 8, 3 and 4) showed moderate percentage of inhibition respectively at concentration 20 μg/ml while the remaining three compounds (5, 2 and 6) showed weak inhibitory activity respectively at same concentration.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
5
v.
1
no.
2013
25
33
https://eajbsg.journals.ekb.eg/article_16642_c3565e275e06b0d1bdd9dcf0f129db73.pdf
dx.doi.org/10.21608/eajbsg.2013.16642
Determination of the Soil Microflora of a Soil Near Microbiology Laboratory at the University of Ilorin Main Campus
Oyeyiola
P.
Department of Microbiology, University of Ilorin, P.M.B 1515, Ilorin, Nigeria
author
Agbaje
B.
Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria
author
Adetunji
O.
Nigerian Stored Products Research Institute, P.M.B 1489, Ilorin, Nigeria
author
text
article
2013
eng
The microflora of a soil near microbiology laboratory at the University of Ilorin, Ilorin, Nigeria was analyzed in this study. Total bacterial count and total fungal count of the soil samples were determined using standard spread plate technique. Six soil samples were collected with interval of two weeks between two samples. Identification of the isolates was done using their colonial, morphological, and biochemical characteristics and established procedures were followed. The total bacterial counts ranged from 1.4 x 108 to 1.7 x 108 cfu/g and the total fungal counts ranged from 1.4 x 105 to 1.5 x 105 cfu/g. The bacteria isolated were Pseudomonas aeruginosa, Staphylococcus epidermidis, Acetobacter species, Lactobacillus species, Erwinia species, Klebsiella species, Bacillus subtilis, Clostridium species, and Bacillus cereus. Isolated fungi were Rhizopus oryzae, Aspergillus niger, Aspergillus flavus, Mucor hiemalis, Penicillium chrysogenum, Fusarium oxysporium, and Trichophyton rubrum.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
5
v.
1
no.
2013
35
41
https://eajbsg.journals.ekb.eg/article_16643_751ae5e278779695bec312ff2784133a.pdf
dx.doi.org/10.21608/eajbsg.2013.16643
Impact of synthetic detergent on sporicidal activity in pharmaceutical facility
Mostafa
Eissa
Department of Microbiological Quality Control, Hikma Pharm Pharmaceutical Company, 6th October City, Giza, Egypt
author
Mohammed
Ashour
Department of Microbiology & Immunology, Faculty of Pharmacy October University, 6th October City, Egypt.
author
Moselhy
Mansy
Department of Microbiology & Immunology, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt
author
text
article
2013
eng
A continuous validation programs for disinfectants are very important in controlling microbial bioburden of environmentally controlled area. Carrier test was chosen because it is more challenging than the usual suspension test. Materials used represent floor, wall, and stainless steel surfaces. This experiment showed that detergents such as sodium dodecyl sulphate improved sporicidal effect on microorganisms even with those who demonstrated relatively high resistance to the used antimicrobial. The used surfactant enhanced the sporicidal activity within five minutes contact time with most commonly found Bacillus speciesin the pharmaceutical facility. However, Bacillus licheniformis spores showed greatest tolerance among studied environmental isolates.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
5
v.
1
no.
2013
43
50
https://eajbsg.journals.ekb.eg/article_16644_14e4a1f3cd1ccb21aa0f0e335b1a3049.pdf
dx.doi.org/10.21608/eajbsg.2013.16644
Biological vs. Chemical Treatments of Agrowastes: Chemical, Physical and Morphological properties
Roushdy
M.
1- Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt
author
Mahdy
Hesham
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt
author
Houssni
El Saied
Cellulose and Paper Department, National Research Centre, Dokki, Cairo 12622, Egypt
author
Altaf
Basta
Cellulose and Paper Department, National Research Centre, Dokki, Cairo 12622, Egypt
author
M.
Hassanin
Cellulose and Paper Department, National Research Centre, Dokki, Cairo 12622, Egypt
author
text
article
2013
eng
i) Aims
The purpose of this study is to improve the surface structure of rice husks, using solid state fermentation (SSF), as eco- treatment process compared to conventional chemical treatments (alkali, and acidic treatments).
ii) Main Methods
A mixed culture of Aspergillus awamori and Cunninghamella elegans in SSF was a proper organisms isolated and used in this investigated treatment. All media used for solid state fermentation were prepared by substituted the carbon source in the foregoing media by RHs fibers, and the incubation period 7 days at 30oC. The beneficial effect of investigated treatment was evaluated by comparing the chemical constituents, IR measurements, thermal stability using non-isothermal thermogravimetric technique, and surface imaging using scanning electron microscope of the treated fibers, with that produced from conventional chemical treated fibers.
iii) Key Findings
The results showed that the investigated biological treatment had positive effect for removing the hemicelluloses and lignin, with preserving the strength of fibers, compared to chemical treated RH. The FTIR- and TGA- measurements, as well as SEM, evident this view.
These results suggest a promising hope that offers the possibility of producing valuable cellulosic material from Egyptian agro-wastes that eventually will be highly amenable for several industries that may improve the Egyptian economy.
iv) Significance
The studies performed should assist future efforts toward applying new biological treatment for RHs fibers, for further application. In addition, application of the extreme processing conditions to traditional one could prove interesting.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
5
v.
1
no.
2013
51
64
https://eajbsg.journals.ekb.eg/article_16645_542d88b1938343150cb71244e21b0a49.pdf
dx.doi.org/10.21608/eajbsg.2013.16645