Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
1
1
2009
12
01
HIV/AIDS’ knowledge and condom usage as preventive measure among university students in South West Nigeria
1
5
EN
Olaitan
O. Lanre,
Lanre
Department of Human Kinetics and Health Education, University of Ilorin, Kwara State Nigeria
10.21608/eajbsg.2009.16712
<span style="font-family: 'Times New Roman','serif'; font-size: 12pt; mso-bidi-language: AR-SA; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: EN-US; mso-bidi-font-style: italic; mso-ansi-language: EN-US;">The study<br />investigated HIV/AIDS knowledge and the use of condom as prevention among the<br />university students in south west Nigeria. A total of 750 students<br />were chosen as subjects using a multistage sampling technique, and a structured<br />questionnaire was used to gather information from the subjects. Research<br />questions were raised and research hypothesis was formulated. Descriptive<br />statistic were used for the socio demographic characteristics, and research<br />question, while the inferential statistics of pearson product moment correlation<br />coefficient was employed to test the hypothesis at 0.05 level of significant.<br />The findings showed that, there existed a significant relationship between the<br />students knowledge of HIV/AIDS and the use of condom as prevention. It was<br />recommended among these that, adequate knowledge about reproductive health,<br />especially sex education should be given priority in our school from secondary<br />and tertiary levels, and government should emphasize on health education as<br />introduce health science back into the secondary school curriculum, because the<br />university students who is expected to pass through this level of education<br />would have gained adequate knowledge about HIV/AIDS and use of condom, since<br />this time usually the experimental stage for sexual activity</span>
HIV/AIDS’,South West Nigeria
https://eajbsg.journals.ekb.eg/article_16712.html
https://eajbsg.journals.ekb.eg/article_16712_c9e060c04a14041d6012d62bb463c47c.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
1
1
2009
12
01
Plasmid mediated virulence factors of some Proteus isolates
7
22
EN
Khaled
Z.
El-Baghdady
Microbiology Department, Faculty of science, Ain Shams Univ., Cairo, Egypt
Mohammad
M.
Abooulwafa
Microbiology & Immunology Department, Faculty. of Pharmacy, Ain Shams Univ., Cairo, Egypt
Madeha
O.
Ghobashy
-Microbiology Department, Faculty of science, Ain Shams Univ., Cairo, Egypt
Hassan
M. G
Gebreel
-Microbiology Department, Faculty of science, Ain Shams Univ., Cairo, Egypt
10.21608/eajbsg.2009.16713
Various<br />virulence factors including invasion, adhesion, cytotoxicity, protease, lipase,<br />elastase, urease production and swarming migration were determined for 24 <em>Proteus</em><br />isolates recovered from clinical specimens. The results showed that the distribution<br />of virulence factors was different among the test isolates and strain specific in<br />most cases. All <em>Proteus</em> isolates showed invasion and adhesion capabilities<br />with different extents. In addition, they were able to produce elastase, urease<br />and exhibit swarming activity. Protease and lipase activities were not detected<br />in any of the isolates. High<br />cytotoxicity was demonstrated in all isolates. A<br />parallel correlation between invasion and cytotoxicity was demonstrated in all<br />isolates. Five isolates of high virulence factors productivities<br />were selected and identified by Analytical<br />Profile Index as <em>Proteus mirabilis</em> (Pr2, Pr12 and Pr24) and <em>Proteus penneri</em> (Pr6 and Pr20).<br />Plasmid curing by acridine<br />orange resulted in the loss of invasiveness<br />and adhesion capabilities of the five<br />isolates, while other virulence factors levels showed no significant<br />difference changes. The results give a clear evidence that both invasion and<br />adhesion of the tested <em>Proteus </em>isolates are plasmid rather than<br />chromosomally encoded.
Proteus,Virulence,invasion,adhesion,urease,plasmid and curing
https://eajbsg.journals.ekb.eg/article_16713.html
https://eajbsg.journals.ekb.eg/article_16713_97fe7cad799b4ab15b51a3d46718e9d6.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
1
1
2009
12
01
Bioaccumulation of heavy metals by Vibrio alginolyticus isolated from wastes of Iron and Steal Factory, Helwan, Egypt
23
28
EN
Hoda
H.
El-Hendawy
Botany Department, Helwan University
Dena
A.
Ali
Microbiology Department, Ain Shams University
Enas
H.
El-Shatoury
Microbiology Department, Ain Shams University
Samah
M.
Ghanem
Botany Department, Helwan University
10.21608/eajbsg.2009.16714
The isolation of bacteria resistant to heavy metals is a topic of interest in the field bioremediation of contaminated water, soil and sediments. We report here the isolation of bacteria that is resistant to high concentration of a mixture of heavy metals namely cadmium, cupper, lead and zinc. The bacterial isolate was obtained from a site receiving heavy metal waste from the iron and steal factory; a major factory located in El-tebeen, south Helwan. The isolate was identified as <em>Vibrio alginolyticus</em> using the API system<strong>. </strong>The maximum tolerable concentration was 2.5 mM, 4 mM, 2.5 mM and 3.5 mM for cadmium, copper, lead and zinc respectively. Transmission electron micrograph of <em>Vibrio alginolyticus</em> grown in nutrient broth containing a mixture of the four tested heavy metals, showed bioaccumulation of heavy metal(s) on the bacterial cell wall. At the same time, there was an over all reduction in the concentration of heavy metals in culture supernatant; the percentage reduction was 20% for cadmium, 31% for cupper 40% for lead and 45%for zinc. The reduction occurred after 4 hrs incubation at 30°C for all metals, cupper, lead, and zinc while cadmium required 24 hrs incubation were required to achieve maximum reduction. This isolate could be used to accelerate the <em>in situ</em> bioremediation of sites contaminated by loads of mixed metals.
Heavy metals,Helwan,Egypt
https://eajbsg.journals.ekb.eg/article_16714.html
https://eajbsg.journals.ekb.eg/article_16714_0ec01dadfea8313a6a502f034d021967.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
1
1
2009
12
01
Effect of biofertilizers and organic phosphorus amendments on growth and essential oil of marjoram (Majorana hortensis L.)
29
36
EN
Ismail
A.
El-Ghandour
Atomic Energy Authority, Nuclear Research Center, Department of Soil and Water
Egypt
Enayat
M.
Desouky
Al-Azhar University, Faculty of Science (Girls Branch), Botany (Microbiology) Department, Egypt
Yehia
G.M.
Galal
Atomic Energy Authority, Nuclear Research Center, Department of Soil and Water
Egypt
Rawahia
A.
Arafa
Al-Azhar University, Faculty of Science (Girls Branch), Botany (Microbiology) Department, Egypt
Abeer
M.M.
Abou Seer
Atomic Energy Authority, Nuclear Research Center, Department of Soil and Water
Egypt
10.21608/eajbsg.2009.16715
The effect of bacterial inoculation (biofertilization) and application of faba bean straw and sheep manure considered by the authors to be organic phosphorus sources to marjoram plants that cultivated in field experiment of Atomic Energy Authority, Inshas, Egypt was studied. Faba bean straw and sheep manure were added at rate of 1500 kg P/ ha of both residues. Residues were incorporated into the soil one month prior to marjoram cultivation. Marjoram (root cutting) was inoculated with either <em>B. polymixa</em> and/ or <em>Bradyrhizobium</em> <em>sp.</em> .The results showed that, growth parameter of marjoram plants were positively affected by bacterial inoculation as well as organic phosphorus sources at three cuts. The herb and oil yield were the highest in case of the combination between sheep manure and <em>B. polymixa</em> at the 2<sup>nd</sup> cut than in control. Nutrient uptake of marjoram plants positively responded to bacterial inoculation and the concerned organic phosphorus sources. The chemical composition of marjoram essential oil did not change due to the bacterial inoculation or applied residues, but the percentages of certain constituents were affected.
B. polymixa,Bradyrhizobium sp,marjoram,Organic phosphorus
https://eajbsg.journals.ekb.eg/article_16715.html
https://eajbsg.journals.ekb.eg/article_16715_389a6b5e12ab79597fe71ae5bf6bf337.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
1
1
2009
12
01
Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients
37
47
EN
Samia
Abdou
Girgis
Clinical pathology Depart. Faculty of medicine, Ain shams university
Adel
Ahmed
El-Mehalawy
Microbiology depart., Faculty of Science, Ain SHAMS UNIVERSITY
Lamia
Mohammed
Rady
Ain SHAMS SPECIALIZED HOSPITAL, AIN SHAMS UNIVERSITY
10.21608/eajbsg.2009.16716
The main objective of the present study was to compare between culture based method (Hicrome Candida Differential Agar) and non-culture based method (PCR) for detection and identification of Candida spp. (<em>C.</em> <em>albicans</em>, <em>C. glabrata</em>, <em>C. tropicalis</em> and <em>C. </em><em>parapsilosis</em>) isolated from intensive care unit (ICU) patients of New Kasr El Aini Medical School Educational Hospital, Egypt. The study was carried out during the period August 2008 to September 2009. The results showed that Candida isolates that isolated from Hicrome Candida Differential Agar plates were positive with PCR technique as follow: 100, 95.0, 85.0 and 90.0 for <em>C.</em> <em>albicans</em>, <em>C. glabrata</em>, <em>C. tropicalis</em> and <em>C. </em><em>parapsilosis, </em>respectively. This means that using of Hicrome Candida Differential Agar media for detection and enumeration of Candida spp. was highly sensitive, rapid and economic method.
Nosocomial fungal infections,Candida spp
https://eajbsg.journals.ekb.eg/article_16716.html
https://eajbsg.journals.ekb.eg/article_16716_d39e1e98e1b38f0cc866573b0a3c9cb5.pdf