2024-03-28T16:21:17Z
https://eajbsg.journals.ekb.eg/?_action=export&rf=summon&issue=3495
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0872
2017
9
2
Submerged Fermentation of Jatropha curcas Seedcake in Production of Itaconic Acid by Aspergillus niger and Aspergillus terreus
Patricia
OMOJASOLA
Patience
OKWECHIME
Inthis study, two fungi: Aspergillus niger (ATCC16404) and Aspergillus terreus (ATCC20542) were used to ferment Jatrophaseed cake (JSC) by submerged fermentation for the production of itaconic acid.The physico-chemical analysis of JSC was determined. JSC was shelled, defattedand used as substrate in mineral salts media and the inocula of A. niger and A. terreus for eleven days at pH 5.42, 29±2°C, 10%substrate and 2 ml inocula (2.6 x 108 spores/ml A. niger) (3.7 x 108 spores/ml A. terreus). Carboxylmethylcellulose (CMC) was used as control.Optimization experiments were conducted by varying fermentation parameters.Results of the physico-chemical analysis revealed carbohydrate 16.23%; protein29.3%; fibre 10.42%; fat 32.13%; ash 5.75% and moisture 6.15%. Itaconic acidyields of 154.0 g L-1 and 208.0 g L-1 were produced by A. niger and A. terreus respectively. Results of the optimization showed higheryields of itaconic acid by A. niger to209.0 g L-1 at pH 2.5, 3 ml inocula, 25% substrate, at 26±2°C on Day 8 and A. terreus yielded of 218.0 g L-1 at pH 3.5,4ml inocula, 25% substrate at 29±2° C on Day 8. These resultssupport potential of JSC for industrial production of itaconic acid.
Aspergillus niger
Aspergillus terreus
fermentation
Jatropha curcas
Itaconic acid
2017
08
01
1
9
https://eajbsg.journals.ekb.eg/article_16329_cc64019350ed5931085f9050a853e44f.pdf
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0872
2017
9
2
Studying of Physico-chemical and Biological characters of Qarun Lake, El-Fayoum – Egypt
El-Sayed
M.
Mosad
A.
LakeQarun is an inland lake occupies the lowest part of El- Fayoum depression. Thesampling program during the studied period was carried out on monthly basisfrom May 2015 and continued till April 2016 (12 successive months).Physico-chemical analysis of Qarun Lake revealed that pH values recorded wereon the alkaline side, values of Secchi disc indicated that the water of thelake concerned as a turbid water bodies. The alkalinity in Qarun Lake ischaracterized by increasing of bicarbonate values as compared with carbonateone. Nutrients analysis indicated increasing of ammonium, nitrate, nitrite,phosphate, silicate in the water of the lake especially in front of the drains.On the other hand a total of 89 species of phytoplankton belonging to sixclasses were recorded in the lake. The recorded classes were Bacillariophyceae,Cyanophyceae, Chlorophyceae, Euglenophyceae, Dinophyceae and Cryptophyceae,.TheBacillariophyceae were most diverse with 39 species, then Cyanophyceae with 18species, Chlorophyceae and Euglenophyceae with 10 species for both, Dinophyceaewith 9 species and Cryptophyceae with 3 species. Our study concluded that theincreased nutrient level in the lake by time show an exacerbated problem ofeutrophication which lead to economic effect on fish production
Qarun Lake
physico-chemical
Parameters
phytoplankton
2017
08
01
11
20
https://eajbsg.journals.ekb.eg/article_16331_7f180012b547a0941b9c3513343221e2.pdf
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0872
2017
9
2
Cotton Aphid (Aphis gossypii Glover) and Green Peach Aphid (Myzus persicae Sulzer) Efficiency of Zucchini Yellow Mosaic Virus Potyvirus (ZYMV) transmission on Squash Plants At Fayoum Governorate.
Hamada
Abd El-Wareth
Hoda
Ahmed
Zucchiniyellow mosaic virus (ZYMV) is an important non- persistent virus causing high squashproduction losses in Egypt. Aphis gossypii Glover and Myzus persicaeSulazer insects have high efficiency of ZYMV transmission from infected squashplants to healthy ones in Egypt,in advance of their rapid spreading. ZYMV was isolated fromnaturally infected squash plants collected from two locations (Sennoris &Fayoum), at Fayoum governorate. Virus identification was done byserological tests (ELISA) using four different antiserums. Virus wastransmitted to healthy squash plants by mechanical transmission and by using thetwo aphid insects. Fecundity and life span of two aphids andforms (alate and apterous) were studied. ELISA test had relay that the viruswas ZYMV. Typical symptoms of virus were observed by mechanical and aphidinsect's inoculation,yellowmosaic, necrosis, leaf curling, blisters stunting, deformation, reduction inleaf size and knobbed fruits (sever malformation).Fecundity and life span ofAphis gossypii were higher than thoseof Myzus persicae on infectedsquash plantscompared with healthy, respectively.The reducing aphid insects population isrecommended in order to decreasing losses caused by ZYMV infection (as theirability of transmission), in addition of removing the infected plantscontinuously which considered as a source if virus inoculum
Aphis gossypii
Myzus persicae
zucchini yellow mosaic virus
squash plants
2017
08
01
21
29
https://eajbsg.journals.ekb.eg/article_16332_4ddb40ca45a93d3b5c0963d5ae0c126f.pdf
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0872
2017
9
2
Alkaliphilic Actinomycetes I.Isolation, Identification and Some Characterization
Asmaa
El-Hosainy
Monira
Zahran
Shadia
Sabry
Five strains of alkaliphilic actinomycetes were isolated from Al-Fayoum region soil, Egypt. Four strains A3, A6, A8, and A10 were classified as genus Kitasatospora on the basis of morphological and chemotaxonomic characteristics. These strains produced aerial mycelium consisting of chains of 20 or more smooth-surface spores. Whole-cell hydrolysates contained both LL- and meso-DAP (diaminopimilic) acid and galactose. Alkaline media were favored for growth, production of aerial mycelium and soluble yellow-brownish pigments. All these strains may be considered as new species for the genus Kitasatospora. The fifth strains contained neither meso-nor-LL-DAP (diaminopimilic acid) also it didn’t contain any diagnostic sugar in the whole-cell hydrolysate. The colonial growth of strain A11 was beige beaver yellow-brownish. It may be considered as a new genus. All isolated strains had a temperature range of 25-40°C and pH range (7-12). Lecithinase; protease and lipase enzymes were secreted by all strains on neutral and alkaline egg emulsion agar medium. All strains reduced nitrate degraded gelatin, casein, skimmed milk, starch, tween 60, DNA, Urea, L-tyrosine and hypoxanthine.
Extremophiles
Alkaliphilic
Actinomycetes
isolation
identification
Characterization
2017
08
01
31
54
https://eajbsg.journals.ekb.eg/article_68790_789456e8b6fdaa1b29466595b6bcafb0.pdf