PCR has been commonly used for genomic viral diagnosis for its sensitivity and accuracy. It showed a higher sensitivity when compared to virus isolation in tissue culture and also in antigenemia detection. Definitely, DNA samples are critical factor in PCR validity. Out of 84 serum samples subjected to this study and extracted by Wizard® DNA purification mini kit, 27 samples (32.1%) were positive PCR. While, 15 samples (17.9%) only out of the same population study (84) were positive PCR for HCMV DNA in native serum samples (without DNA extraction). This result was confirmed the importance of DNA extraction from serum samples for detection of HCMV which, subsequently lead to more sensitive diagnostic tool of an ongoing HCMV infection.
Shoman, S., Tabl, A., Ghanem, H., & Nabil, M. (2010). Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2(1), 1-8. doi: 10.21608/eajbsg.2010.16711
MLA
Sahar Shoman; Ashraf Tabl; Hussam Ghanem; Mohamed Nabil. "Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples". Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2, 1, 2010, 1-8. doi: 10.21608/eajbsg.2010.16711
HARVARD
Shoman, S., Tabl, A., Ghanem, H., Nabil, M. (2010). 'Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples', Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2(1), pp. 1-8. doi: 10.21608/eajbsg.2010.16711
VANCOUVER
Shoman, S., Tabl, A., Ghanem, H., Nabil, M. Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2010; 2(1): 1-8. doi: 10.21608/eajbsg.2010.16711
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