Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2

Abdallah, Omnia Mohamed; EL-Baghdady, Khaled Z.; Khalil, Mostafa M.H.; El Borhamy, Mervat I.; Meligi, Gamal

doi:10.21608/eajbsg.2019.55269

Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2

Document Type : Original Article

Authors

¹ Department of Microbiology, Faculty of Dentistry, Misr International University, Cairo, Egypt

² Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt

³ Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt

⁴ -Department of Microbiology, Faculty of Pharmacy, Misr International University, Cairo, Egypt -International Medical Center, Clinical Microbiology Laboratory, Cairo, Egypt

10.21608/eajbsg.2019.55269

Abstract

Green biosynthesis of selenium nanoparticles (SeNPs) using Bacillus tropicus Ism 2 (MK332444) isolated from soil polluted with industrial wastewater near the Ismailia Canal, Egypt, was carried out. SeNPs were biogenically synthesized using bacterial pellets mixed with 1 mM Na₂SeO₃. Characterization of biosynthesized SeNPs was carried out by UV-Vis spectrum where SeNPs solution exhibited characteristic peak at 270 nm. Dynamic Light Scattering (DLS) analysis and High-Resolution Transmission Electron Microscope (HRTEM) showed spherical shaped SeNPs with size ranged from 60 to 125 nm with PDI= 0.544 and zeta potential value of -30.6. Biologically synthesized SeNPs showed no antibacterial activity against both Gram-positive and Gram-negative bacteria. Cytotoxicity of SeNPs was studied against Huh-7 human liver cell line and showed low cytotoxicity with IC₅₀value of367±5.5 μg/ml. In conclusion in this work Bacillus tropicus Ism 2 locally isolated bacterium showed the capability to biosynthesize SeNPs with spherical shape and low cytotoxicity.

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