Fatty Acid Composition of Eight Isolates of Entomopathogenic Nematodes from Five Egyptian Governorates
Meligy
A.
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
author
Azazy
M.
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
author
Sorour
A.
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
author
Monzer
A .
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
author
text
article
2017
eng
Thefatty acid composition of infective juveniles (IJs) related to five isolates ofHeterorhabditis indica (EGAZ1, EGAZ2, EGAZ3, EGAZ4, and EGAZ5) and threeisolates of Steinernema carpocapsae (EGAZ9, EGAZ10 and SA)collected from five Egyptian Governorates was assessed. Also, fatty acidcomposition of IJs from two commercially relevance strains of H.bacteriophora (HP88)and S. carpocapsae (All), was examinedfor comparison. Newly emerged IJs of all isolates had fatty acid number andpattern similar to that of the corresponding commercial species. Of the tenfatty acids identified, oleic, was the main fatty acid in all species andisolates. Unsaturated fatty acids were dominant and total amount of saturatedfatty acids of H. indica isolates was significantly higher than that of S.carpocapsae isolates. Palmitic was the second most abundant fatty acidin IJs of H. indica isolates, while linoleic was the second mostabundant fatty acid in IJs of S. carpocapsae isolates. Ofthe tested Egyptian isolates, IJs of EGAZ3 and EGAZ5 of H. indica hadthe highest amounts of fatty acids and their contents of saturated fattyacids/gram body weight are comparable to that of the commercial strain. It is suggested that isolates EGAZ3 and EGAZ5of H. indica are the candidates for developing practical Egyptianbio-control product based on nematode formulation.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
9
v.
3
no.
2017
1
8
https://eajbsg.journals.ekb.eg/article_16322_b7786f4479f60ee0b1eadcc818ed574a.pdf
dx.doi.org/10.21608/eajbsg.2017.16322
Distribution and identification of potato cyst nematodes from Ain Defla region, Algeria
Tirchi
N.
University Djilali Bounaama of Khemis Miliana, Ain Defla, Algeria
author
Mokabli
A.
University Djilali Bounaama of Khemis Miliana, Ain Defla, Algeria
author
Troccoli
A.
Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, Italia
author
De Luca
F.
Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, Italia
author
Fanelli
E.
Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, Italia
author
text
article
2017
eng
Potato cyst nematodes PCNs are the most economically damaging pest of potato crop worldwide. During 2013, a survey was carried out in Ain Defla region of Algeria. 81 soil samples collected from potato fields of 14 localities were submitted to nematological analysis which revealed the presence of these nematodes in 22.22% of the prospected fields. Sixteen PCN populations from five localities were characterized by combination of features the perineal regions of cysts and those of second stage juveniles. The morphological identification has been confirmed by the analysis of the ITS-RFLP profiles, sequencing and phylogenetic analysis of the ITS region. The results revealed that the two species Globodera rostochiensis and G. pallida are present in this region occurring separately or in mixed populations. However, we noted a dominance of G. pallida since only 12.25% of the populations have been identified like G. rostochiensis whereas 31.5% were G. pallida and 56, 25% of the populations were constituted of a mixture of the two species and among these mixed populations, 77.77% presented a dominance of G. pallida. The predominance of G. pallida has been noted in the sites of Ain Defla, El Amra, Mekhatria and Arib. G. rostochiensis was dominant in Rouina. Intraspecific variation was noted between populations of G. rostochiensis and G. pallida. Because of the high divergence among Algerian populations of G. pallida and G. rostochiensis it can be assumed that they were multi-introduced in Algeria. The most divergent population of G. pallida, that formed a well separated group with some populations from Chile and Peru, suggests a later or independent introduction of this population in Algeria.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
9
v.
3
no.
2017
9
20
https://eajbsg.journals.ekb.eg/article_16323_bbc55ad517e07c0b6a6d3e0aad53daca.pdf
dx.doi.org/10.21608/eajbsg.2017.16323
Antimicrobial Activities of Some Actinomycete Strains Isolated from the Sinai Egypt Soils
Abdel-Rahman
G.
Plant Protection Department, Desert Research Center, Mataria, Cairo, Egypt
author
B.
Refaat
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt
author
M.
Helal
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
author
A.
Kobisi
Plant Protection Department, Desert Research Center, Mataria, Cairo, Egypt.
author
text
article
2017
eng
Seventy three pure actinomycete colonies were isolatedfrom 48 rhizospheric soil samples revealing different locations in Sinai. Theseisolates were tested for their antimicrobial activities against Bacillussubtilis and Staphylococcus aureus as gram-positive bacteria, Escherichiacoli and Pseudomonas aeruginosa as gram-negative bacteria, Candidaalbicans as an unicellular fungi and Aspergillus niger, Aspergillusflavus, Rhizoctonia solani and Fusarium oxysporum asfilamentous fungi. Isolates S6, S13 and S35 were the most effectiveagainst the tested bacteria and fungi. They had the lowestMinimum Inhibitory Concentration (MIC) values and showedthe highest and widest spectrum of antibacterial and antifungal activities. Factorscontrolling actinomycetes active metabolite(s) productivity were studied.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Egyptian Society of Biological Sciences
2090-0872
9
v.
3
no.
2017
21
31
https://eajbsg.journals.ekb.eg/article_16325_150de8b8c12d22a43feabc31314f7a17.pdf
dx.doi.org/10.21608/eajbsg.2017.16325