@article { author = {Lanre, Olaitan}, title = {HIV/AIDS’ knowledge and condom usage as preventive measure among university students in South West Nigeria}, journal = {Egyptian Academic Journal of Biological Sciences, G. Microbiology}, volume = {1}, number = {1}, pages = {1-5}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {2090-0872}, eissn = {2090-0880}, doi = {10.21608/eajbsg.2009.16712}, abstract = {The studyinvestigated HIV/AIDS knowledge and the use of condom as prevention among theuniversity students in south west Nigeria. A total of 750 studentswere chosen as subjects using a multistage sampling technique, and a structuredquestionnaire was used to gather information from the subjects. Researchquestions were raised and research hypothesis was formulated. Descriptivestatistic were used for the socio demographic characteristics, and researchquestion, while the inferential statistics of pearson product moment correlationcoefficient was employed to test the hypothesis at 0.05 level of significant.The findings showed that, there existed a significant relationship between thestudents knowledge of HIV/AIDS and the use of condom as prevention. It wasrecommended among these that, adequate knowledge about reproductive health,especially sex education should be given priority in our school from secondaryand tertiary levels, and government should emphasize on health education asintroduce health science back into the secondary school curriculum, because theuniversity students who is expected to pass through this level of educationwould have gained adequate knowledge about HIV/AIDS and use of condom, sincethis time usually the experimental stage for sexual activity}, keywords = {HIV/AIDS’,South West Nigeria}, url = {https://eajbsg.journals.ekb.eg/article_16712.html}, eprint = {https://eajbsg.journals.ekb.eg/article_16712_c9e060c04a14041d6012d62bb463c47c.pdf} } @article { author = {El-Baghdady, Khaled and Abooulwafa, Mohammad and Ghobashy, Madeha and Gebreel, Hassan}, title = {Plasmid mediated virulence factors of some Proteus isolates}, journal = {Egyptian Academic Journal of Biological Sciences, G. Microbiology}, volume = {1}, number = {1}, pages = {7-22}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {2090-0872}, eissn = {2090-0880}, doi = {10.21608/eajbsg.2009.16713}, abstract = {Variousvirulence factors including invasion, adhesion, cytotoxicity, protease, lipase,elastase, urease production and swarming migration were determined for 24 Proteusisolates recovered from clinical specimens. The results showed that the distributionof virulence factors was different among the test isolates and strain specific inmost cases. All Proteus isolates showed invasion and adhesion capabilitieswith different extents. In addition, they were able to produce elastase, ureaseand exhibit swarming activity. Protease and lipase activities were not detectedin any of the isolates. Highcytotoxicity was demonstrated in all isolates. Aparallel correlation between invasion and cytotoxicity was demonstrated in allisolates. Five isolates of high virulence factors productivitieswere selected and identified by AnalyticalProfile Index as Proteus mirabilis (Pr2, Pr12 and Pr24) and Proteus penneri (Pr6 and Pr20).Plasmid curing by acridineorange resulted in the loss of invasivenessand adhesion capabilities of the fiveisolates, while other virulence factors levels showed no significantdifference changes. The results give a clear evidence that both invasion andadhesion of the tested Proteus isolates are plasmid rather thanchromosomally encoded.}, keywords = {Proteus,Virulence,invasion,adhesion,urease,plasmid and curing}, url = {https://eajbsg.journals.ekb.eg/article_16713.html}, eprint = {https://eajbsg.journals.ekb.eg/article_16713_97fe7cad799b4ab15b51a3d46718e9d6.pdf} } @article { author = {El-Hendawy, Hoda and Ali, Dena and El-Shatoury, Enas and Ghanem, Samah}, title = {Bioaccumulation of heavy metals by Vibrio alginolyticus isolated from wastes of Iron and Steal Factory, Helwan, Egypt}, journal = {Egyptian Academic Journal of Biological Sciences, G. Microbiology}, volume = {1}, number = {1}, pages = {23-28}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {2090-0872}, eissn = {2090-0880}, doi = {10.21608/eajbsg.2009.16714}, abstract = {The isolation of bacteria resistant to heavy metals is a topic of interest in the field bioremediation of contaminated water, soil and sediments. We report here the isolation of bacteria that is resistant to high concentration of a mixture of heavy metals namely cadmium, cupper, lead and zinc. The bacterial isolate was obtained from a site receiving heavy metal waste from the iron and steal factory; a major factory located in El-tebeen, south Helwan. The isolate was identified as Vibrio alginolyticus using the API system. The maximum tolerable concentration was 2.5 mM, 4 mM, 2.5 mM and 3.5 mM for cadmium, copper, lead and zinc respectively. Transmission electron micrograph of Vibrio alginolyticus grown in nutrient broth containing a mixture of the four tested heavy metals, showed bioaccumulation of heavy metal(s) on the bacterial cell wall. At the same time, there was an over all reduction in the concentration of heavy metals in culture supernatant; the percentage reduction was 20% for cadmium, 31% for cupper 40% for lead and 45%for zinc. The reduction occurred after 4 hrs incubation at 30°C for all metals, cupper, lead, and zinc  while cadmium required  24 hrs incubation were required to achieve maximum reduction. This isolate could be used to accelerate the in situ bioremediation of sites contaminated by loads of mixed metals.}, keywords = {Heavy metals,Helwan,Egypt}, url = {https://eajbsg.journals.ekb.eg/article_16714.html}, eprint = {https://eajbsg.journals.ekb.eg/article_16714_0ec01dadfea8313a6a502f034d021967.pdf} } @article { author = {El-Ghandour, Ismail and Desouky, Enayat and Galal, Yehia and Arafa, Rawahia and Abou Seer, Abeer}, title = {Effect of biofertilizers and organic phosphorus amendments on growth and essential oil of marjoram (Majorana hortensis L.)}, journal = {Egyptian Academic Journal of Biological Sciences, G. Microbiology}, volume = {1}, number = {1}, pages = {29-36}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {2090-0872}, eissn = {2090-0880}, doi = {10.21608/eajbsg.2009.16715}, abstract = {The effect of bacterial inoculation (biofertilization) and application of faba bean straw and sheep manure considered by the authors to be organic phosphorus sources to marjoram plants that cultivated in field experiment of Atomic Energy Authority, Inshas, Egypt was studied. Faba bean straw and sheep manure were added at rate of 1500 kg P/ ha of both residues. Residues were incorporated into the soil one month prior to marjoram cultivation. Marjoram (root cutting) was inoculated with either B. polymixa and/ or Bradyrhizobium sp. .The results showed that, growth parameter of marjoram plants were positively affected by bacterial inoculation as well as organic phosphorus sources at three cuts. The herb and oil yield were the highest in case of the combination between sheep manure and B. polymixa at the 2nd cut than in control. Nutrient uptake of marjoram plants positively responded to bacterial inoculation and the concerned organic phosphorus sources. The chemical composition of marjoram essential oil did not change due to the bacterial inoculation or applied residues, but the percentages of certain constituents were affected.}, keywords = {B. polymixa,Bradyrhizobium sp,marjoram,Organic phosphorus}, url = {https://eajbsg.journals.ekb.eg/article_16715.html}, eprint = {https://eajbsg.journals.ekb.eg/article_16715_389a6b5e12ab79597fe71ae5bf6bf337.pdf} } @article { author = {Girgis, Samia and El-Mehalawy, Adel and Rady, Lamia}, title = {Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients}, journal = {Egyptian Academic Journal of Biological Sciences, G. Microbiology}, volume = {1}, number = {1}, pages = {37-47}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {2090-0872}, eissn = {2090-0880}, doi = {10.21608/eajbsg.2009.16716}, abstract = {The main objective of the present study was to compare between culture based method (Hicrome Candida Differential Agar) and non-culture based method (PCR) for detection and identification of Candida spp. (C. albicans, C. glabrata, C. tropicalis and C. parapsilosis) isolated from intensive care unit (ICU) patients of New Kasr El Aini Medical School Educational Hospital, Egypt. The study was carried out during the period August 2008 to September 2009. The results showed that Candida isolates that isolated from Hicrome Candida Differential Agar plates were positive with PCR technique as follow: 100, 95.0, 85.0 and 90.0 for C. albicans, C. glabrata, C. tropicalis and C. parapsilosis, respectively. This means that using of Hicrome Candida Differential Agar media for detection and enumeration of Candida spp. was highly sensitive, rapid and economic method.}, keywords = {Nosocomial fungal infections,Candida spp}, url = {https://eajbsg.journals.ekb.eg/article_16716.html}, eprint = {https://eajbsg.journals.ekb.eg/article_16716_d39e1e98e1b38f0cc866573b0a3c9cb5.pdf} }