Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
9
3
2017
12
01
Fatty Acid Composition of Eight Isolates of Entomopathogenic Nematodes from Five Egyptian Governorates
1
8
EN
Meligy
A.
A.
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
Azazy
A.
M.
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
Sorour
H.
A.
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
Monzer
M.
A .
Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research Center
10.21608/eajbsg.2017.16322
The<br />fatty acid composition of infective juveniles (IJs) related to five isolates of<br /><em>Heterorhabditis indica</em> (EGAZ1, EGAZ2, EGAZ3, EGAZ4, and EGAZ5) and three<br />isolates of <em>Steinernema carpocapsae</em> (EGAZ9, EGAZ10 and SA)<br />collected from five Egyptian Governorates was assessed. Also, fatty acid<br />composition of IJs from two commercially relevance strains of <em>H.<br />bacteriophora </em>(HP88)and<em> S. carpocapsae</em> (All), was examined<br />for comparison. Newly emerged IJs of all isolates had fatty acid number and<br />pattern similar to that of the corresponding commercial species. Of the ten<br />fatty acids identified, oleic, was the main fatty acid in all species and<br />isolates. Unsaturated fatty acids were dominant and total amount of saturated<br />fatty acids of <em>H. indica</em> isolates was significantly higher than that of <em>S.<br />carpocapsae</em> isolates. Palmitic was the second most abundant fatty acid<br />in IJs of <em>H. indica </em>isolates, while linoleic was the second most<br />abundant fatty acid in IJs of <em>S</em>. <em>carpocapsae</em> isolates. Of<br />the tested Egyptian isolates, IJs of EGAZ3 and EGAZ5 of <em>H. indica</em> had<br />the highest amounts of fatty acids and their contents of saturated fatty<br />acids/gram body weight are comparable to that of the commercial strain. It is suggested that isolates EGAZ3 and EGAZ5<br />of <em>H. indica </em>are the candidates for developing practical Egyptian<br />bio-control product based on nematode formulation.
Entomopathogenic nematode,Heterorhabditis indica,Heterorhabditis bacteriophora,Steinernema carpocapsae,fatty acid
https://eajbsg.journals.ekb.eg/article_16322.html
https://eajbsg.journals.ekb.eg/article_16322_b7786f4479f60ee0b1eadcc818ed574a.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
9
3
2017
12
01
Distribution and identification of potato cyst nematodes from Ain Defla region, Algeria
9
20
EN
Tirchi
N.
University Djilali Bounaama of Khemis Miliana, Ain Defla, Algeria
Mokabli
A.
University Djilali Bounaama of Khemis Miliana, Ain Defla, Algeria
Troccoli
A.
Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, Italia
De Luca
F.
Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, Italia
Fanelli
E.
Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, Italia
10.21608/eajbsg.2017.16323
Potato cyst nematodes PCNs are the most economically damaging pest of potato crop worldwide. During 2013, a survey was carried out in Ain Defla region of Algeria. 81 soil samples collected from potato fields of 14 localities were submitted to nematological analysis which revealed the presence of these nematodes in 22.22% of the prospected fields. Sixteen PCN populations from five localities were characterized by combination of features the perineal regions of cysts and those of second stage juveniles. The morphological identification has been confirmed by the analysis of the ITS-RFLP profiles, sequencing and phylogenetic analysis of the ITS region. The results revealed that the two species <em>Globodera rostochiensis</em> and <em>G.</em> <em>pallida</em> are present in this region occurring separately or in mixed populations. However, we noted a dominance of <em>G. pallida </em>since only 12.25% of the populations have been identified like <em>G. rostochiensis</em> whereas 31.5% were <em>G. pallida</em> and 56, 25% of the populations were constituted of a mixture of the two species and among these mixed populations, 77.77% presented a dominance of <em>G. pallida.</em> The predominance of <em>G. pallida</em> has been noted in the sites of Ain Defla, El Amra, Mekhatria and Arib. <em>G. rostochiensis</em> was dominant in Rouina. Intraspecific variation was noted between populations of <em>G. rostochiensis</em> and <em>G. pallida</em>. Because of the high divergence among Algerian populations of <em>G. pallida</em> and <em>G. rostochiensis</em> it can be assumed that they were multi-introduced in Algeria. The most divergent population of <em>G. pallida</em>, that formed a well separated group with some populations from Chile and Peru, suggests a later or independent introduction of this population in Algeria.
PCN,Distribution,identification,Ain Defla,Algeria
https://eajbsg.journals.ekb.eg/article_16323.html
https://eajbsg.journals.ekb.eg/article_16323_bbc55ad517e07c0b6a6d3e0aad53daca.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0880
9
3
2017
12
01
Antimicrobial Activities of Some Actinomycete Strains Isolated from the Sinai Egypt Soils
21
31
EN
Abdel-Rahman
A.
G.
Plant Protection Department, Desert Research Center, Mataria, Cairo, Egypt
ag_rahman@outlook.com
B.
M.
Refaat
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt
M.
Helal
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
A.
A.
Kobisi
Plant Protection Department, Desert Research Center, Mataria, Cairo, Egypt.
10.21608/eajbsg.2017.16325
<strong>Seventy three pure actinomycete colonies were isolated<br />from 48 rhizospheric soil samples revealing different locations in Sinai. These<br />isolates were tested for their antimicrobial activities against <em>Bacillus<br />subtilis</em> and <em>Staphylococcus aureus</em> as gram-positive bacteria, <em>Escherichia<br />coli</em> and <em>Pseudomonas aeruginosa</em> as gram-negative bacteria, <em>Candida<br />albicans</em> as an unicellular fungi and <em>Aspergillus niger</em>, <em>Aspergillus<br />flavus</em>, <em>Rhizoctonia solani</em> and <em>Fusarium oxysporum</em> as<br />filamentous fungi. Isolates S6, S13 and S35 were the most effective<br />against the tested bacteria and fungi. They had the lowest<br />Minimum Inhibitory Concentration (MIC) values and showed<br />the highest and widest spectrum of antibacterial and antifungal activities. Factors<br />controlling actinomycetes active metabolite(s) productivity were studied. <br /></strong>
antimicrobial activities. Actinomycet. Sinai Egypt
https://eajbsg.journals.ekb.eg/article_16325.html
https://eajbsg.journals.ekb.eg/article_16325_150de8b8c12d22a43feabc31314f7a17.pdf