2024-03-28T11:39:05Z
https://eajbsg.journals.ekb.eg/?_action=export&rf=summon&issue=3517
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0872
2016
8
2
Biological Activity of Different Botanical Origin of Propolis
Asmaa
Fawzy
Mai
Al-Deeb
The present study was designed to investigate the effect of antimicrobial ethanolic extract of propolis (EEP) on gram negative and gram positive bacterial. The tested bacterial strains were Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Proteus as Gram negative, while Staphylococcus aureus and Streptococcus pyogenes consider as Gram positive. Four different types of propolis (Saudi, Turkish, Chinese and Egyptian) were used in this study. There was highly significant effect of Saudi, Egyptian and Turkish propolis on tested bacteria. The highest inhibition zone in Egyptian propolis was 12 mm with Proteus mirabilis at 10% concentration while the lowest inhibition zone was 1.6 mm with E.coli at 1.25% concentration at the same propolis. Proteus mirabilis showed the highest inhibition diameter which record 14 mm at 10% concentration in Saudi propolis while the lowest diameter was recorded as 2.6 mm at 1.25% concentration with P.aeruginosa. In Chinese propolis the Proteus showed the highest inhibition zone at 1.25 %, 2.50%, 5%, 7.50% and 10% concentration that recorded (5mm), (7.3mm), (9.6mm), (11mm) and (13 mm) respectively. In Turkish propolis the 10.5 mm was recorded as the highest inhibition zone at 7.5% and 10% concentration in Klebsiella pneumoniae, on the other hand, the lowest inhibition zone in S.aureus was 1.6 mm at 1.25% concentration. the antibacterial activity of propolis was concentration depends and depends upon its botanical origin
Propolis
Antibacterial
S. aureus
S. pyogenus
E. coli
Proteus mirabilis
P. aeruginosa
Klebsiella pneumoniae
2016
12
01
1
9
https://eajbsg.journals.ekb.eg/article_16470_ae727a67789dc2b86efc6cb26122daec.pdf
Egyptian Academic Journal of Biological Sciences, G. Microbiology
2090-0872
2090-0872
2016
8
2
Incidence of Human Cytomegalovirus Viremia among Egyptian Hepatitis C - Patients with Hepatocellular Carcinoma
Ahmed
Khedr
Marwa
Ibrahim
Ahmed
Barakat
Mohamed
Salama
Kouka
Abdel-wahab
Mostafa
El-Awady
Background: Hepatocellular carcinoma (HCC) is the major hepatic complication that may arise after many years of hepatitis C virus (HCV) infection. In Egypt, HCV represents the major health problem, also human cytomegalovirus (HCMV) is known as one of the highest un-resolved latent infections among general population. HCMV viremia in the co-infection with HCV may cause life threatening in HCC patients. Our study aimed to detect HCMV viremia in HCV–patients experienced HCC, and to investigate its role in disease worsening. Methods: In HCC patients, HCV-RNA viral load was determined by real- time polymerase chain reaction. In HCV-HCC patients, HCMV-DNA was detected by amplification of gB gene region using nested- polymerase chain reaction. Results: HCMV-DNA was detected in 4/73 in control subjects with prevalence rate of 5.4%, whereas HCMV–DNA was recorded in 24/75 HCV-HCC patients with prevalence rate of 32 %. Data on the level of alpha feto protein (AFP) was available for 59 out of 75 HCV-HCC patients, this enabled us to differentiate between low risk HCC group of 40/59 patients with AFP < 500 ng/ml, from them HCMV- DNA was detected in 14/40 patients with prevalence rate 35%, and high risk HCC group of 19/59 patients with AFP > 500 ng /ml, from them HCMV-DNA was reported in 9/19 patients with prevalence rate 47.37%. High significant prevalence rate of HCMV-DNA (P < 0.001) among control and HCC subjects was reported. Significant change in HCMV – DNA prevalence between low and high risk HCC groups could not be achieved but tendency of higher prevalence rate in HCMV- DNA was observed towards HCC high risk group of patients. Conclusion: we illustrated information about HCMV/ HCV co-infection in HCC patients, which referred to the association of HCMV viremia with HCV-HCC patients, as well as the tendency of elevation in HCMV viremia from low to high risk HCC patients depending on AFP threshold of 500 ng/ml.
human cytomegalovirus
Hepatitis C virus
Hepatocellular carcinoma
2016
12
01
11
21
https://eajbsg.journals.ekb.eg/article_16471_52f7d1da2cc8f2ba00af8e1756d4b073.pdf