Egyptian Society of Biological SciencesEgyptian Academic Journal of Biological Sciences, G. Microbiology2090-08729320171201Fatty Acid Composition of Eight Isolates of Entomopathogenic Nematodes from Five Egyptian Governorates181632210.21608/eajbsg.2017.16322ENMeligy A.A.Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research CenterAzazy A.M.Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research CenterSorour H.A.Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research CenterMonzer M.A .Pest Physiology Dept., Plant Protection Research Institute (PPRI), Agricultural Research CenterJournal Article20181011The<br />fatty acid composition of infective juveniles (IJs) related to five isolates of<br /><em>Heterorhabditis indica</em> (EGAZ1, EGAZ2, EGAZ3, EGAZ4, and EGAZ5) and three<br />isolates of <em>Steinernema carpocapsae</em> (EGAZ9, EGAZ10 and SA)<br />collected from five Egyptian Governorates was assessed. Also, fatty acid<br />composition of IJs from two commercially relevance strains of <em>H.<br />bacteriophora </em>(HP88)and<em> S. carpocapsae</em> (All), was examined<br />for comparison. Newly emerged IJs of all isolates had fatty acid number and<br />pattern similar to that of the corresponding commercial species. Of the ten<br />fatty acids identified, oleic, was the main fatty acid in all species and<br />isolates. Unsaturated fatty acids were dominant and total amount of saturated<br />fatty acids of <em>H. indica</em> isolates was significantly higher than that of <em>S.<br />carpocapsae</em> isolates. Palmitic was the second most abundant fatty acid<br />in IJs of <em>H. indica </em>isolates, while linoleic was the second most<br />abundant fatty acid in IJs of <em>S</em>. <em>carpocapsae</em> isolates. Of<br />the tested Egyptian isolates, IJs of EGAZ3 and EGAZ5 of <em>H. indica</em> had<br />the highest amounts of fatty acids and their contents of saturated fatty<br />acids/gram body weight are comparable to that of the commercial strain. It is suggested that isolates EGAZ3 and EGAZ5<br />of <em>H. indica </em>are the candidates for developing practical Egyptian<br />bio-control product based on nematode formulation.Egyptian Society of Biological SciencesEgyptian Academic Journal of Biological Sciences, G. Microbiology2090-08729320171201Distribution and identification of potato cyst nematodes from Ain Defla region, Algeria9201632310.21608/eajbsg.2017.16323ENTirchiN.University Djilali Bounaama of Khemis Miliana, Ain Defla, AlgeriaMokabliA.University Djilali Bounaama of Khemis Miliana, Ain Defla, AlgeriaTroccoliA.Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, ItaliaDe LucaF.Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, ItaliaFanelliE.Istituto per la Protezione Sostenibile delle Piante-CNR di Bari, ItaliaJournal Article20181011Potato cyst nematodes PCNs are the most economically damaging pest of potato crop worldwide. During 2013, a survey was carried out in Ain Defla region of Algeria. 81 soil samples collected from potato fields of 14 localities were submitted to nematological analysis which revealed the presence of these nematodes in 22.22% of the prospected fields. Sixteen PCN populations from five localities were characterized by combination of features the perineal regions of cysts and those of second stage juveniles. The morphological identification has been confirmed by the analysis of the ITS-RFLP profiles, sequencing and phylogenetic analysis of the ITS region. The results revealed that the two species <em>Globodera rostochiensis</em> and <em>G.</em> <em>pallida</em> are present in this region occurring separately or in mixed populations. However, we noted a dominance of <em>G. pallida </em>since only 12.25% of the populations have been identified like <em>G. rostochiensis</em> whereas 31.5% were <em>G. pallida</em> and 56, 25% of the populations were constituted of a mixture of the two species and among these mixed populations, 77.77% presented a dominance of <em>G. pallida.</em> The predominance of <em>G. pallida</em> has been noted in the sites of Ain Defla, El Amra, Mekhatria and Arib. <em>G. rostochiensis</em> was dominant in Rouina. Intraspecific variation was noted between populations of <em>G. rostochiensis</em> and <em>G. pallida</em>. Because of the high divergence among Algerian populations of <em>G. pallida</em> and <em>G. rostochiensis</em> it can be assumed that they were multi-introduced in Algeria. The most divergent population of <em>G. pallida</em>, that formed a well separated group with some populations from Chile and Peru, suggests a later or independent introduction of this population in Algeria.Egyptian Society of Biological SciencesEgyptian Academic Journal of Biological Sciences, G. Microbiology2090-08729320171201Antimicrobial Activities of Some Actinomycete Strains Isolated from the Sinai Egypt Soils21311632510.21608/eajbsg.2017.16325ENAbdel-Rahman A.G.Plant Protection Department, Desert Research Center, Mataria, Cairo, EgyptB. M.RefaatBotany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, EgyptM.HelalBotany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.A. A.KobisiPlant Protection Department, Desert Research Center, Mataria, Cairo, Egypt.Journal Article20181011<strong>Seventy three pure actinomycete colonies were isolated<br />from 48 rhizospheric soil samples revealing different locations in Sinai. These<br />isolates were tested for their antimicrobial activities against <em>Bacillus<br />subtilis</em> and <em>Staphylococcus aureus</em> as gram-positive bacteria, <em>Escherichia<br />coli</em> and <em>Pseudomonas aeruginosa</em> as gram-negative bacteria, <em>Candida<br />albicans</em> as an unicellular fungi and <em>Aspergillus niger</em>, <em>Aspergillus<br />flavus</em>, <em>Rhizoctonia solani</em> and <em>Fusarium oxysporum</em> as<br />filamentous fungi. Isolates S6, S13 and S35 were the most effective<br />against the tested bacteria and fungi. They had the lowest<br />Minimum Inhibitory Concentration (MIC) values and showed<br />the highest and widest spectrum of antibacterial and antifungal activities. Factors<br />controlling actinomycetes active metabolite(s) productivity were studied. <br /></strong>