Multifaceted Potentialities of Some Rhizobacteria Associated With Sorghum Plants on Their Growth and Development

Document Type : Original Article

Authors

1 Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt

2 Desert Research Center, El-Matariya, 11753, Cairo, Egypt

Abstract

The present study deals with the potential of rhizobactria isolated from soils rhizosphere associated with Sorghum [Sorghum bicolor (L.) Moench] roots. The isolates were characterized for production of indole-3-acetic acid (IAA), phosphate solubiliztion ability and some lytic enzyme activity as functional potentialities correlated with plant growth promoting activities. Consequently, the isolates were identified by 16S ribosomal RNA by sequencing analysis. The result appeared that most isolates produced IAA and the highest amount of IAA was detected from Bacillus megaterium which produced 0.453 µg/ml and Pseudomonas hibiscicola that produced 0.370 µg/ml.
Sorghum plants inoculated with selected rhizobacterial strains were significantly enhanced specially by Pseudomonas geniculat (SC), Rhizobium pusense (SD), Bacillus cerues ATCC 14579 (S4) and Bacillus cerues strain X3 (S2) Lysinibacillus sp(S3).  Whereas, the mineral contents (Mn, Fe, Cu, Zn, N, P and K) were significantly higher values in the shortest plant shoot and lowers in the higher shoot of sorghum plants. Meanwhile, sorghum parameters of photosynthetic pigments, amino acids and N, P, and K concentrations were increased significantly as compared with its untreated control. Consequently, the more efficient isolates were identified as Rhizobium pusense, Bacillus cerues strain X3 and Bacillus cerues ATCC 14579 respectively. Further, the isolate may be used as plant growth promoting rhizobacteria and could use as abiocontrol agents based on the production of lytic enzymes like protease, amylase, lipase and chitinase which are the key enzyme for lyses fungal cell wall.
It could be concluded that the isolated strains have the ability for production of phytohormons and phosphate solubilization which can be used as abiofertilizers due to enhancing the tested crop plant growth parameters. Finally the rhizospheric isolated strains could used as bioinculant to increase plant tolerance against biotic and a biotic stress and providing a step forward toward sustainable agriculture.

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