Genotypic Identification and Evaluation of Several Selective Media for Recovery of Aeromonas spp. from Different Sources

Document Type : Original Article

Authors

1 Departement of Bacteriology, Immunology & Mycology Faculty. Vet Med. Suez Canal University

2 Animal Health Research Institute. Port Said Lab Hygiene

3 Clinical Pathology Department, Faculty of Medicine

Abstract

A   total number of 250 samples of tilapia Niloticus fish (50), mugil cephalus   fish (50), drinking water (25 tap water in addition to 25 bottled mineral   water), pond water (50) and childhood diarrhea (50) were collected from Suez   canal area and these samples were cultured on several selective media, The   isolation rate of Aeromonas spp. from all samples using enrichment   technique  on starch ampicillin agar   (SAA), Rimler-shotts medium (RS), Blood ampicillin agar (BAA) & MacConkey   ampicillin agar (MAA) were (51.2%), (45.2%), (38.8%) and (31.6%)   respectively. The incidence and distribution of Aeromonads from different   sources of 250 samples (tilapia Niloticus fish, mugil cephalus fish,  drinking tap water, bottled mineral water,   pond water and childhood diarrhea were 44 (88%), 33 (66%), 4 (16)%, 0 (0%),   42 (84%) and 5 (10%) respectively. The total number of  Aeromonas isolates from 250 examined samples   were 260 isolates that were biochemically identified into 4 biotypes as 136   (52.31%) A. hydrophila, 81 (31.15%) A.   sobria, 34 (13.08%) A. caviae and 9 (3.46%) A. schubertii.   The highest prevalence isolated of Aeromonas spp. were A. hydrophila   followed by A. sobria, A. caviae and A. schubertii.   Results of antibiogram of isolated Aeromonas spp. demonstrated that all   tested Aeromonas isolates were resistant to Erythromycin,   Sulphamethoxazol-Trimethoprim beside Ampicillin, and while the highest degree   of sensitivity towards Ciprofloxacin, Norfloxacin, Amikacin and Gentamicin.   Molecular typing of Aeromonas isolates by Polymerase chain reaction technique   to determine of 16SrRNA gene that present in all examined isolates of  Aeromonas were (100%), and 2 virulence   genes were found: aerolysin gene by a percentage of (83.3%) ,and hemolysin   gene by a percentage of (8.3%) which present only in  A. schubertii.

Keywords

Files

Share

How to cite

Statistics