Isolation and Identification of Mycoflora Contaminated of Yemeni Coffee Beans

Document Type : Original Article

Authors

Biology Department, Faculty of Science, Sana'a University, Sana'a, Yemen

Abstract

This
work was designed to study the mycoflora of stored Yemeni green and roasted
coffee beans, in 50 samples (25 samples each) which were collected randomly
from different markets in
Sana'a city, Yemen during 2013 using direct
plating technique. Our results showed that in the individual samples of green
and roasted coffee beans, the counts of fungi were ranging from 110 to 236 CUF
/ 100 beans and 8 to 249 CUF/100 beans, respectively. As well the broadest
spectrum of fungal species were 55 species and 3 varieties belonging to 13
genera and 66 species and 3 varieties belonging to 23 genera in case of green
and roasted coffee beans, respectively. Aspergillus, Penicillium, and
Rhizopus
followed by Fennelliaand Fusarium were the most
common genera isolated from both the two types of coffee beans samples, whereas
Eurotium
was the most common genus only in roasted coffee bean samples,
while it was absent in green coffee beans samples.

Keywords