Characterization and Identification of Moderately Thermophilic Bacteria Isolated from Jazan Hot Springs in Saudi Arabia

Document Type : Original Article

Authors

Department of Biological Sciences, College of Science, King Khalid University, Saudi Arabia

Abstract

Three
new moderately thermophilic bacteriawere isolated from three (Al-Khawbah, Bani Malik, and
Al-Bozah)
hot springs located in Jazan district, south-western of Saudi Arabia.The isolates
were identified by the sequence analysis of the 16S rRNA gene. The 16S rRNA
gene sequence alignment with GenBank resulted in a percent identity (% ID) with
closest GenBank match of 98% suggesting that the isolates are new strains
within the branching of well-defined genus Bacillus. The phylogenetic analysis
of these strains using their 16S rDNA sequence data showed that strains SA and
SS30 had highest homology (98%) with B. licheniformis, whereas strain
SA09 showed 98% similarity with B. subtilis. The morphological,
biochemical, and physiological characteristics
of the isolates were also studied. They were aerobic, gram-positive, rod-shaped,
and moderately thermophilic bacteria (with an optimum growth temperature of
45–50˚C, and pH of 6).

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