Document Type : Original Article
Authors
1
-Department of Biotechnology, center of chemistry and chemical engineering, Lund university, Sweden -Biotechnology Research Center (BRC), Suez Canal University, (New Campus), Ismailia 41522, Egypt
2
-Biotechnology Research Center (BRC), Suez Canal University, (New Campus), Ismailia 41522, Egypt -Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
3
-Department of Marine Biology, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt -Department of Biology, Almadinah Almonawara, Tybah university, Kingdom of Saudi Arabia
4
-Department of Marine Biology, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt -Department of Food Technology, faculty of Agriculture, Suez Canal University, Ismailia 41522,, Egypt
5
Biotechnology Research Center (BRC), Suez Canal University, (New Campus), Ismailia 41522, Egypt
Abstract
A successful 17 β estradiol (E2) degrading bacterium was isolated from waste water of Saft Elhena drain in Egypt. Molecular identification of the isolated bacteria was carried out using DNA (RAPD), the amplification was made by restriction analysis of amplified 16s rDNA and sequencing. Alos, the bacterium was incubated in saline media under different levels of 17 β-estradiol anaerobically. Levels of CO2 production were examined by GC and residual 17 β-estradiol was tested by HPLC to measure biodegradation capacity. Identification of the genetic element responsible of the gene resistance in the bacteria was tested. The sequence analysis of 16S rRNA gene for 17 β estradiol degrader bacterium showed that its affiliations to phylum Enterobacteriaceae, and it belonged to the genera Klebsiella sp. Results revealed that Klebsilla was cabable of degrading estradiol and that the responsible resistance gene was mostly the chromosomal gene. However, further studies are still going on to locate and examine the gene responsible for E2 resistance.
Keywords
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