First Isolation, In Egypt, of a granulovirus (AgseGVEG) from Agrotis segetum Schiffermuller (Lepidoptera: Noctuidae)

Document Type : Original Article

Author

Plant Protection Research Institute, ARC, Dokki, Giza, Egypt Department of Cutworms and Mole Cricket

Abstract

A new Granulovirus was isolated from cutworm Agrotis segetum larvae collected from the field in Qalubaya governorate.
Transverse section of larvae showed infected fat body, epidermis and mid-gut cells.
Transmition electromicroscope evealed ovoid occlusion bodies (granules), each granule contains a single rod-shaped virion with one nucleocapsid, typical of granuolosis virus. Also, the scanning electromicroscopy of apurified viral suspension confirmed a baculovirus morphology. The size of virus particles in the present isolation ranged between 1.9 mm to 2.5mm. Preliminary bioassay indicated that A. ipsilon neonate larvae are susceptible to AgseGVEG and the calculated LC50 value was 3.79x106capsules/ml.               

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