This study aimed to determine antimycolic acid antibodies [IgG and IgM] among TB/HIV Co-infected patients in Khartoum State. Sputum and blood specimens were collected from patients attended Alsha'ab Teaching Hospital, Tuberculosis Reference Laboratory, Ibrahim Malik Hospital and Abu Anga Hospital; patients were all informed and consented. Direct smears from sputum samples of 90 suspected patients showed that 17 (18.9%) were acid fast bacilli positive while 73 (81.1%) were negative. The ninety sputum specimens were subjected to PCR to amplify IS6110 region specific for Mycobacterium tuberculosis complex. The result showed that 79 (87.8%) were positive for IS6110 while 11 (12.2%) were negative. The 90 serum samples were investigated for HIV using dot plot technique, 9 samples (10%) were found HIV positive and they were all TB positive by PCR. 80 Serum samples were analyzed by ELISA, 16 (20%) gave positive result for antimycolic acid IgG while 64(80%) were negative and 55 (68.8%) were positive for antimycolic IgM, while 25 (31.3%) were negative. When HIV infection was correlated with specific antibodies for antimycolic acids, in 6 HIV positive samples, one (17 %) was positive and 5 (83 %) were negative for IgG, while 2 (33.3 %) were positive and 4 (67 %) were negative for IgM. This study concluded that patients with TB/HIV Co-infection have less capability of producing antimycolic acids antibodies, however, IgM antibodies could be of more serodiagnostic value.
Elhassan, M., Elhassan, O., Dirar, A., Abbas, E., & Elmekki, M. (2011). Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 3(1), 27-32. doi: 10.21608/eajbsg.2011.16692
MLA
Mogahid M Elhassan; Osama A. Elhassan; Ahmed E. Dirar; Elrayah E. Abbas; Miskelyemen A. Elmekki. "Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan", Egyptian Academic Journal of Biological Sciences, G. Microbiology, 3, 1, 2011, 27-32. doi: 10.21608/eajbsg.2011.16692
HARVARD
Elhassan, M., Elhassan, O., Dirar, A., Abbas, E., Elmekki, M. (2011). 'Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan', Egyptian Academic Journal of Biological Sciences, G. Microbiology, 3(1), pp. 27-32. doi: 10.21608/eajbsg.2011.16692
VANCOUVER
Elhassan, M., Elhassan, O., Dirar, A., Abbas, E., Elmekki, M. Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2011; 3(1): 27-32. doi: 10.21608/eajbsg.2011.16692
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