Paclitaxel and Its Chemo-preventive Role in the Management of Small Lung Cancer

Eltayeb, M. Fikry; Helmy, Mohamed; Arouk, M.; Ibrahim, Talat; Khattab, Abeer A.

doi:10.21608/eajbsg.2021.171342

Paclitaxel and Its Chemo-preventive Role in the Management of Small Lung Cancer

Document Type : Original Article

Authors

¹ Botany and Microbiology Department, Faculty of Science, Benha University, Qalubiya Governorate 13511, Egypt.

² Cancer biology Department, South Egypt Cancer Institute – Assiut University, Egypt.

³ Botany Department, Faculty of Science, Zagazig University, Egypt

10.21608/eajbsg.2021.171342

Abstract

Based on a quantitative analysis using HPLC and UV, Alternaria alternata has been identified as a strong taxol producer. Taxol's biosynthetic efficiency has been checked using PCR detection.The development of taxol by A. alternata was investigated by inoculating the fungus on various media.The best medium for extracting the highest volume of taxol was found to be potato dextrose broth (PDB). After 21 days of incubation at 23°C with a pH of 8.5 and shaking (120 rpm) (250 g/L), the substance taxol was formed to perfection. HPLC was used to effectively purify the Alternaria alternata taxol. The taxol from Alternaria alternata displayed promising anticancer properties. The capacity of anticancer drugs to eradicate cancer cells may be used to determine their efficacy. As a result, the inhibitory effect of taxol on A549 lung adenocarcinoma cells was assessed by culturing the cells with different concentrations of the compound for 24 hours (MTT assay).