Optimization of Cultural Conditions to Maximize the Efficiency of Petroleum Crude Oil-Degrading Bacteria

Document Type : Original Article

Authors

1 Agricultural Microbiology Dept., National Research Center, Cairo, Egypt

2 Microbiology Dept., Faculty of Science, Ain Shams University, Egypt

Abstract

The
optimization of cultural conditions to maximize the efficiency of petroleum
crude oil bioremediation by three bacterial strains, Alcaligenes faecalis,
Microbacterium oxydans and Microbacterium Paraoxydans were
isolated from Tebbin, Al Kanater charity and Agiba, respectively. The mineral
salt medium was used, pH 7.5, temperature 30 oC and 30 days
of incubation showed the highest degradation of crude oil with the isolated
three bacterial strains. Ammonium sulphate as a sole N-source and crude oil as
a sole C-sources were more suitable for crude oil degradation at the
concentration of 0.2 % and 1.0 %, respectively. Inoculum size of 0.8 % was the
most suitable inoculum for the degradation. Moreover, the agitation condition
was advantages at 100 rpm than the static condition for the crude oil
degradation by the three bacterial strains. 

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