Analysis of Different Bioactive Compounds Conferring Antimicrobial Activity from Lactobacillus plantarum and Lactobacillus acidophilus with Gas Chromatography-Mass Spectrometry (GC-MS)

Document Type : Original Article

Authors

1 Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

2 Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt

3 Microbiology and Immunology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.

Abstract

The chemical screening tests are important tools for the detection of bioactive principles. In this study, gas chromatography−mass spectrometry (GC−MS) was successfully used to identify the biochemical constituents produced by Lactobacillus plantarum and Lactobacillus acidophilus strains. The method is based on liquid-liquid extraction followed by gas chromatography-mass spectrometry (GC-MS) analysis. The GC-MS chromatogram of the extracts of L. plantarum and L. Acidophilus revealed the presence of twenty-two and seventeen chemical compounds respectively with antimicrobial activities. Bioactive compounds as Pentadecanoic acid, Butanoic acid, Eucalyptol, Isopropyl Myristate possess, and 9-Hexadecenoic acid were detected in the extract of L. plantarum. Also, other compounds as 1- Tetradecanol, Isochiapin B, cis-Vaccenic acid, 1,2-Benzenedicarboxylic acid were detected for L. acidophilus. Furthermore, different bioactive compounds with antimicrobial activities as Oleic acid, 1-Hexadecanol, 2-methyl, 2,2- Dideutero octadecanal, 9-Octadecenoic acid were detected in both extracts of L. plantarum and L. Acidophilus. Thus, these probiotics are strong candidates as antimicrobial agents.

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