The Influence of The Isolated Enterobacter spp and Pantoea sp., on Barley’s Phosphorus Uptake Grown in Calcareous Soil

Sabra, Mayada A; Ahmed, Abdallah E.

doi:10.21608/eajbsg.2022.221870

The Influence of The Isolated Enterobacter spp and Pantoea sp., on Barley’s Phosphorus Uptake Grown in Calcareous Soil

Document Type : Original Article

Authors

Mayada A Sabra ¹
Abdallah E. Ahmed ²

¹ Agriculture Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, Egypt

² Soil and Water Technologies Dept., Arid Land Cultivation Research Institute, the City of Scientific Research and Technological Applications (SRTA-city). New Borg El-Arab, Alexandria, Egypt

10.21608/eajbsg.2022.221870

Abstract

The purpose of this study was to determine the possibility of three phosphate-releasing bacteria (PSB) increasing the bioavailability of phosphorus (P) in a calcareous soil fertilized with single superphosphate (SSP) and its absorption by the barley (Hordeum vulgare L.) variety Giza 123. The pot experiment, a trial using calcareous soil, was conducted at the experimental farm located in Borg Al-Arab. The inoculated and uninoculated barley seeds were sown on December 1^st, 2020 for 60 days. The bacterial isolates were Enterobacter aerogenes(ENPSB 1), Pantoea sp. (ENPSB 2), Enterobacter sp < /em>. (ENPSB 3), and their mixture in the ratio (1:1:1) was tested in combination with four levels of single super phosphate SSP (0%, 50%, 75%, and 100% of the recommended dosage for barley (150 kg/Fed.). In a randomized full-block design, each treatment included three replicates. Treatments and their interactions significantly affected the plant's dry weight, and bioavailable P in the soil. When these PSB strains were inoculated together (in a mixed culture), they were able to operate synergistically and were responsible for an increase in plant growth, P absorption, and accessible P in the soil when compared to a single inoculation. Also, plants inoculated with the different PSB isolates had a significant impact on total amino acids as compared with un-inoculated plants. Accordingly, we can reduce SSP application to 75% of the recommended dosage + inoculation with a mixed culture of these PSB to have a major impact on barley growth more than the application of 100% SSP of the recommended dosage without PSB inoculation to maintain environmental and soil health.

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