Document Type : Original Article
Authors
1
Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt
2
Oral and maxillofacial surgery in Benha, Benha University Hospitals, Egypt.
Abstract
This study aimed to isolate and characterize bacterial dental caries and for this purpose, 60 samples were collected from people with caries Tooth decay for the period from the beginning of May 2018 to the end of September 2020, for both sexes, at different ages ranging from (7-60) years, Then they were collected and transported directly to the laboratory and the samples were planted by plotting on plates of tryptic soy blood agar anaerobic conditions, Only 13 specimen showed a positive result with (21.7%) percentage, the most common bacteria which responsible for dental cavities are Streptococcus mutans, Staphylococcus aureus and Enterococcus faecalis. Here, three pathogenic bacteria S. aureus, S. mutans and E. faecalis were identified by Bergey’s Manual of Systematic Bacteriology, Antibiotic sensitivity patterns of the isolated pathogenic bacteria were assessed against the selection of antibiotics. these types of bacteria have been shown high resistant to different antibiotics like Erythromycin, Methicillin, Oxacillin, Cephalexin, Aztreonam, Amikacin, Gatifloxacin, Chloramphenicol, Gentamicin, Norfloxacin, Pencillin-G and Ceftazidime while showed sensitivity to some antibiotics like Trimethoprim-sulfamethoxazole, Ciprofloxacin, Amoxicillin, Levofloxacin, Nitrofurantoin and Vancomycin, and using antimicrobial agents, as a biocontrol agent, against certain antibiotic-resistant bacteria causing dental caries, To overcome this problem, by applying different strain of probiotics e.g., Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus plantarrum, Lactobacillus rhamnosus, Enterococcus faecalis, Bacillus subtilis, Yeast, and Lacteolfort capsules were applied against the three pathogenic bacteria which showed growth inhibition of these isolates , the zone of inhibition has high efficiency in case of Lactobacillus spp than Bifidobacterium spp even though against those with antibiotic-resistant pattern. There was another propagation used to overcome the bacterial dental caries which was mouthwashes propagation e.g., chlorohexidine, which showed high efficiency in the case of S. aureus then S. mutans and E. faecalis, this study refers that the isolated probiotics and mouthwashes are promising biocontrol agents that could challenge antibiotic-resistant dental caries bacteria to announce new successful alternatives to antibiotics.
Keywords
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