Isolation and Diagnosis of Biofilms of Klebsiella pneumoniae Bacteria and Candida albicans Yeast, and Studying the Sensitivity of The Pathogens to Antibiotics and Antifungals

Abdulla, Sara N.; Dheeb, Batol I.; AL-Qaysi, Safaa AL-Deen A. S.; Farhan, Mohammed S S.; Massadeh, Muhannad; Hamid, Abbas Fadhel

doi:10.21608/eajbsg.2023.320271

Isolation and Diagnosis of Biofilms of Klebsiella pneumoniae Bacteria and Candida albicans Yeast, and Studying the Sensitivity of The Pathogens to Antibiotics and Antifungals

Document Type : Original Article

Authors

¹ Department Of Pathological Analysis, College of Applied Sciences, University of Samarra.

² Department of Biology, College of Sciences (For Women), University of Baghdad.

³ Department of Biology, college of Sciences, University of Tikrit.

⁴ Dept. Of Biology and Biotechnology, Faculty of Sciences, 13115 The Hashemite University.

⁵ Ministry of health, Samarra general hospital.

10.21608/eajbsg.2023.320271

Abstract

Section describes the details of a laboratory study that was conducted between September 2022 and February 2023. The study was carried out at the laboratories of the Department of Life Sciences, College of Science, University of Baghdad, and the University of Al-Nahrain Center/Al-Nahrain University. The highest percentage of antibiotic resistance was found in K. pneumoniae isolates against the antibiotic Cefopime, which reached 66.67%. Meanwhile, the highest percentage of antifungal resistance was found in C. albicans isolates against the antifungal Metronidazole, which reached 73.33%. Yeast isolates (C.albicans, C.glabrata, C.kefyr, C.krusei, C.parapsilosis, C.tropicalis, C.lusitaniae) were diagnosed and tested for their ability to form biofilms on Congo red agar medium, where 40 isolates were found to be biofilm producers, with a rate of 40%.(ESBL) gene indicating an increase in the rate of antibiotic resistance in Gram-negative bacteria and their occurrence in inanimate hospital environments.

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