Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30

Abd-El Razik, Mohamed; El-Waseif, Amr A.; Abobaker, Rabea A.; Emam, Ferial M.; Hassan, Mervat G.

doi:10.21608/eajbsg.2024.399897

Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30

Document Type : Original Article

Authors

¹ Botany and Microbiology Dept., Faculty of Science, Suez Canal University, Egypt.

² Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.

³ Botany and Microbiology Dept., Faculty of Science, Banha University, Egypt.

10.21608/eajbsg.2024.399897

Abstract

Probiotic developments recently have provided new avenues for the fight against cancer. Via making use of Lactobacillus brevis extract's reducing properties. Therefore, the current study investigated whether nano-exopolysaccharide (NEPS) from probiotic can decrease cell proliferation, increase apoptosis, and activate autophagy in CaCo2 and HT-29 cells using in vitro techniques. On the other hand, compared to non-apoptotic cells, apoptotic cells showed orange-colored bodies as a result of cellular shrinkage and hemorrhage brought on by NEPS stimulation. Not to be overlooked is the NEPS 's exceptional selectivity in causing cell death, particularly in CaCo2 and HT-29 cells. For CaCo2 and HT-29 cells, the half-maximum inhibitory concentration (IC50) values were 9.4 μg/ml and 6.2 μg/ml, respectively. In HT-29 cells, probiotics cause an increase in the synthesis of cytokines linked to inflammation, such as TNF-β, IL-8, and IL-6. As an outcome of apoptosis being triggered and immune system responses being strengthened, our study's findings suggest that using probiotics could be a practical approach to eliminating colorectal malignant cells.

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